Force Curve - Update Sensitivity
NanoScope software allowws for the updating of sensitivity on Force Curvespreviously captured files. The goal of updating the sensitivity of a Force Curve is to calibrate the Y axis (Cantilever Deflection for Contact Mode or Amplitude for Tapping Mode).
Update Sensitivity Procedure
- When updating sensitivity it is important to use the Z Sensor channel (Height Sensor) if available and to set the Active Curve to Retract. When placing cursors, it is preferred to place them such that they are over a linear section of the plot (not too close to the pull off or peak force). Additionally, select as long of a section as possible.
NOTE: When using the Update Sensitivity function, it is assumed that there is no deformation of the tip or sample. This requires a hard sample for the results to be valid.
- After the Force Curve file has already been opened and the cursors placed, click the Update Sensitivity icon or select Plot > Update Sensitivity from the Menu Bar, as shown in Figure 1.
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- The NanoScope Analysis window, show in , will appear. Choose Yes to update the sensitivity.
- The sensitivity is updated in the Force Curve window, as seen below.
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